Clostridium botulinum type C in the Mersey estuary

Structure and toxicity of Clostridium botulinum type C toxin.

chromatography on a column of 9AE Sephadex A-50 in the presence of 5% of 2mercaptoethanol at OOC. No toxic activity was detected on both the chains. They were different from each other in antigenecity, amino acid composition and electric charge. Reassociation of these two components gave a molecule with iower texic act{vity (O.1-3% of the native toxin) in the delay of S-S linkage formation, whe...

Purification and crystallization of Clostridium botulinum type C toxin.

Double Sandwich ELISA Modified Method for the Detection of Clostridium Botulinum Type E

Background & Objective: A very small amount of botulinum toxin can cause death and on the other hand, there is no cure for its poison other than antitoxin. Therefore, a diagnostic method that can detect very small amounts of botulinum toxin in a short time is very important. In this study, rapid and accurate detection of botulinum toxin type E has been performed with the double sandwich ELISA m...

Immunogenic and Protective Potentials of Recombinant Receptor Binding Domain and a C-Terminal Fragment of Clostridium botulinum Neurotoxin Type E

Clostridium Botulinum Type E neurotoxin heavy chain consists of two domains: the translocation domain asthe N-terminal half and the binding domain as the Cterminal half (Hc). One effective way to neutralize botulinum neurotoxin is to inhibit binding of this toxin to neuromuscular synapses with antibodies against binding domain. Two synthetic genes, coding for Hc (the full length binding d...

Clostridium botulinum Type D Lysogenized Organisms

We examined production of ADP-ribosyltransferase C3 in 11 strains of Clostridium botldinum type C and D and their nontoxigenic derivatives. Antisera to C3 proteins of type C organisms divided C3 proteins roughly into at least two groups, bearing no relation to their bacterial types. The C3 gene of type D strain South African was isolated from a toxigenic phage library, and the complete sequence...